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Effects of Tea (Camellia sinensis) or its Bioactive Compounds l-Theanine or l-Theanine plus Caffeine on Cognition, Sleep, and Mood in Healthy Participants: A Systematic Review and Meta-Analysis of Randomized Controlled Trials

Nutrition Reviews systematic review of 50 RCTs (15 contributed to ≥1 random-effects SMD meta-analysis through Aug 2023) reports small-to-moderate acute cognitive/mood signals favouring theanine+caffeine versus placebo in the first two hours post-dose for several tasks, with wide confidence intervals; theanine alone showed a small-moderate choice reaction-time advantage versus placebo in hour 1.

Design

  • Corpus: 50 RCTs in healthy adults; 15 trials entered ≥1 meta-analysis (Aug 2023 search)
  • Interventions: tea, l-theanine, l-theanine + caffeine, vs placebo
  • Endpoints: cognition, mood, sleep (sleep MA coverage is limited vs cognition arms—read forest plots)

Example acute SMDs vs placebo (abstract; hour-specific)

Theanine + caffeine vs placebo

  • Choice reaction time (h1): SMD −0.48 (95% CI −1.01 to 0.05)
  • Digit vigilance accuracy (h2): SMD 0.20 (0.02 to 0.38)
  • Attention-switching accuracy (h2): SMD 0.33 (0.13 to 0.54)
  • Overall mood (h2): SMD 0.26 (−0.10 to 0.63)

Theanine alone vs placebo

  • Choice reaction time (h1): SMD −0.35 (−0.61 to −0.10)

Evidence hygiene

CIs often include null; acute lab tasks long-term clinical benefit; do not merge with titrated depression omega-3 trials or high-dose caffeine sleep work (Gardiner 2023) without reading arm definitions.

Publication

Payne ER, Bain JR, Nussbaumerova M, et al. Nutr Rev. 2025 Oct 1;83(10):1417–1446. PMID 40314930.

Outcomes

  • Effect Size (Cohen's d / SMD)
    -0.35
    d (Cohen's d)
  • Effect Size (Cohen's d / SMD)
    0.33
    d (Cohen's d)
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