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Cold water immersion in recovery following a single bout resistance exercise suppresses mechanisms of miRNA nuclear export and maturation
Within-subject muscle biopsy study in nine young men: after resistance exercise, 10 min cold-water immersion (10°C) versus active recovery suppressed DROSHA and EXPORTIN-5 mRNA at 24–48 h and altered miR-1/miR-133a-related anabolic signaling pathways—mechanistic evidence that CWI can blunt molecular programs linked to muscle adaptation.
Design
- Participants: 9 young men; vastus lateralis biopsies at rest and 2, 4, and 48 h after a single resistance-exercise bout
- Recovery arms: 10 min CWI at 10 °C vs active recovery at ambient temperature
Key molecular contrasts (abstract examples)
- DROSHA / EXPORTIN-5 mRNA: lower after CWI than active recovery at 24 h and 48 h (p values reported ≤ 0.025 for DROSHA; ≤ 0.008 for EXPORTIN-5 at 24 h in abstract)
- Authors link miR-1 / miR-133a–related programs to intracellular anabolic signalling and discuss angiogenic miRNAs (miR-15a, miR-126) alongside PAX7 / NCAM and VEGF readouts
Evidence hygiene
- Acute mechanistic design—supports “CWI may interfere with hypertrophy signalling” narratives adjacent to longer training studies; not a performance or DOMS endpoint meta-analysis.
Publication
D'Souza RF, Figueiredo VC, Markworth JF, et al. Physiol Rep. 2023 Aug 11;11(15):e15784. PMID 37549955.
Outcomes
- Strength Recovery RateVersus active recovery after resistance exercise, 10 min CWI at 10°C suppressed DROSHA and EXPORTIN-5 mRNA at 24–48 h (p≤0.025 and p≤0.008 respectively at highlighted time points; D'Souza et al. 2023).